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Fig. 3. Extracellular pH change demonstrated by Bromocresol Purple (pH 5.2, yellow;
pH 6.8, purple). (A) Alkalinization of extracellular space in the outer zone,
t=1 h, 7 min. The arrow shows a position partially deprived of cells
to show the change in color more clearly. (B,C) A drop of Bromocresol Purple
solution (left) kept at a distance from a 2-D cell mass (right) also changes
color after (B) t=15 min and (C) t=2h, 44 min. Scale bars,
100 µm (A); 1 mm (B,C).
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