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Fig. 5. The effects of the rk mutation on the ability of flies to respond to bursicon or its putative second messenger, cyclic AMP. Neck-ligatured wild-type (wt) flies were injected with (A) an extract containing bursicon or (B) saline. Neck-ligatured rk4 flies were injected with (C) a bursicon extract, (D) saline or (E) 8-Br-cAMP. The arrows indicate the abdominal tergites where darkening has occurred.





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