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Fig. 3. Homozygous rk4 flies show normal release of eclosion
hormone at ecdysis and are responsive to injections of ecdysis triggering
hormone (MasETH). (A,B) The eclosion hormone immunoreactivity in the ventral
nervous system of rk4 flies before (A) and after (B) adult
eclosion. The insets show the loss of large immunoreactive swellings after
eclosion. (C) The latency to adult ecdysis when pharate adult
rk4 homozygotes were injected with MasETH. The advance in
the time to ecdysis is identical to that seen in normal flies. 5 nervous
systems from each stage were assayed.