Fig. 1. The DLGR2 coding region is disrupted in noncomplementing deficiencies that end within the rk gene and in two strong rk alleles. (A) Non-complementing deficiencies, Df(2L)b-L and Df(2L)A376, were mapped by testing for the presence of sequence tagged sites, STS1, STS2 and STS3, in a mixed population of homozygous balancer and homozygous deficiency flies. (B) Df(2L)A376 breaks in the 5' end of DLGR2 removing STS1, the initiator ATG and most of the first exon. (C) Df(2L)b-L removes STS2 and STS3 and breaks in 3' end of the gene removing at least the entire transmembrane domain. 20 embryos from each cross were tested. (D) Electropherogram from a rk¹ homozygote. The wild-type sequence of codon 698, TAC, is converted to TAA, changing a tyrosine residue to a stop codon. (E) In rk⁴ the wild-type sequence of codon 954, TGG, is changed to TGA, altering a tryptophan to a termination codon.

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