Fig. 4. Representative current/voltage (I/V) relationships elicited by free amino acids in 100 mmol l^-1 Na⁺/0 mmol l^-1 K⁺ medium. The I data represent total currents obtained by subtracting control I/V data from the I/V data in the presence of 0.5 mmol l^-1 amino acids for (A) CAATCH1 WT and (B) CAATCH1 Y147F. For A, in the case of methionine, leucine, phenylalanine and glycine, the blockade of inward current is represented by inverted I/V data as a result of the subtraction protocol representing the algebraic inverse of the inhibited current. V_m, membrane potential.

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