Fig. 6. Immunohistochemical localisation of phosphorylated p38-MAPK in the ventricle of isolated amphibian heart perfused under normal conditions (A) and with 50 µmol l^-1 of phenylephrine (PHE) for 0.5 min in the absence (B) or presence of either 1 µmol l^-1 prazosin (C), 1 µmol l^-1 propranolol (D) or 1 µmol l^-1 SB203580 (E). Cryosections were incubated with phospho-p38 MAPK specific antibody (1:200 dilution) and counterstained with Haematoxylin. Representative photographs from three independent experiments performed with similar results are shown. Immunolocalisation deposits are visualised with Fast Red chromogen. Bar, 20 µm.

Return to article