Fig. 5. Effect of - and ß-AR antagonists on p38-MAPK stimulation by isoproterenol (ISO). p38-MAPK phosphorylation was assessed by western immunoblotting in samples (100 µg of protein) from control Rana ridibunda hearts and hearts perfused with 50 µmol l^-1 ISO in the presence or absence of either 1 µmol l^-1 propranolol (PRO) (Ai) or 10 µmol l^-1 prazosin (PZ) (Aii). Western blot analysis was performed as described in Materials and methods. Identical samples were also assayed for total p38-MAPK immunoreactivity (Aiii). Values are means ± S.E.M. Each experiment was repeated on at least two further occasions with similar results. Bands were quantified by laser scanning densitometry and plotted (B). Values significantly different from controls: P<0.001.

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