Fig. 2. Characterisation of antibodies. Triton X-100 extracts of rat normal soleus muscle were subjected to immunoprecipitation with normal (Ø), polyclonal rabbit antisera A-17 against the N terminus (N) or the serum (C) of focal adhesion kinase (FAK) against the C-terminal portion (C) of FAK protein. Equal amounts of the precipitates, together with a sample of cell extract (E), were resolved by SDS—PAGE on 7.5 % gels and immunoblotted with either anti-FAK antiserum C-FAK (A) or anti-phosphotyrosine antibody (pTyr) (B). The sizes of molecular mass markers are indicated.

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