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Fig. 5. Kinetics of frog mitochondrial oxygen consumption rates in the low oxygen
range at 3 °C for hypoxic submerged frogs. The kinetics for state 3 (A)
and state 4 (B) respiration are shown. Solid line, control; dashed line,
1-month-submerged; dotted line, 4-month-submerged groups of frogs. The insets
show how P50 was calculated for each group of frogs. These
curves were generated using the equation
O2=100[PO2/(P50+PO2)],
where
O2 (as a
percentage of maximum) is the rate of oxygen consumption for state 3 or state
4. The state 3 and state 4 values from the control group of frogs were set to
100%, and those for the 1- and 4- month-submerged groups of frogs were
expressed relative to the controls. To convert PO2 to
µmol, kPa must be multiplied by a factor of 11.3 and mmHg by a factor of
1.5. To convert kPa to mmHg, kPa must be multiplied by a factor of 7.51. From
St-Pierre et al. (2000c) with
permission.