Fig. 3. Phylogenetic relationships among the striated MYH genes. The phylogenetic tree is based on neighbor-joining (N-J) analysis of the MYH amino acid sequences aligned in Fig. 2,Fig. 2,Fig. 2,Fig. 2. All gapped positions were omitted for the N-J analysis. Because it was the most divergent of the striated MYHs, the IIM gene was designated as the outlier. The number of times 1000 independently chosen subsequences yielded the same tree are indicated at the node points. This bootstrap analysis indicates that the tree is well supported. A partial human IIM sequence was assembled by analysis of the chromosome 7 DNA sequence using GeneQuest. Because the human IIM genomic sequence is currently incomplete, a full-length IIM sequence was generated by fusing the human sequence to amino acid residues 1-525 of the cat IIM sequence (Hoh et al., 1999). A frameshift we identified in the coding region of the human IIM gene probably explains the absence of IIM expression in human (Rowlerson et al., 1983).

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