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Fig. 1. Expression of Alrp in rat organs and blood samples. Samples (20 µg) of total protein extract were separated in non-reducing 4 % to 12 % gradient gels. The respective western blot was probed with an antibody specific for mammalian Alrp. Proteins were analysed with (+) and without (-) reducing agent (20 mmol l-1 dithiothreitol) in the sample buffer. The molecular masses (MW) of marker proteins are listed in kDa. Three forms of Alrp with molecular masses of 23, 21 and 15 kDa were identified. Under non-reducing conditions, all three forms of Alrp exist as dimers. S, spleen; K, kidney; H, heart, L, lung; C, cerebellum; B, brain; Li, liver; F, fat tissue; M, muscle, T, testis; LB, liver blood; vB, venous blood; aB, arterial blood. DTT, dithiothreitol.





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