Fig. 2. Production of the male-specific compound (24h collection) CPB I by 20
untreated male Colorado potato beetles (N=4) (A) and enhancement of
CPB I production by experimental treatments (1 µl injection into the gas
chromatograph of 50 µl of rinse): (B) topical treatment with 10 µg of
juvenile hormone III (JH III) (N=4); (C) antennectomy (N=3);
and (D) combined treatment of topical application of JH III and antennectomy
(N=3). CPB I, male-specific compound; P, 6-methyl-5-hepten-2-one;
FID, response of flame ionization detector. The electroantennogram (EAD)
response is included for A—C. EADs in A and B were recorded from female
antennae; the EAD in C was recorded from a male antenna. No differences were
observed in EADs of male and female antennae. SR, sesquiterpene region.
