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Fig. 3. Cytosolic calcium [Ca2+]i response of rectal gland artery (RGA) to external Ca2+ and Gd3+ or to spermine. (A) Change in [Ca2+]i of RGA maintained in calcium-free shark Ringer following the addition of Ca2+ (approx. 3 mmol l-1) and then Gd3+ (333 µmol l-1) (values are means ± S.E.M., N=10; *P<0.01 for addition of Ca2+ compared to baseline [Ca2+]i and **P<0.05 for Gd3+ compared to Ca2+). (B) Representative example of the increase in [Ca2+]i of RGA in calcium-free buffer in response to the addition of spermine (333 µmol l-1) at the time indicated by the arrow.





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