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Fig. 4. Example of a 1H-NMR spectrum of a living muscle from a blue crab that had been acclimated to a salinity of 35{per thousand}. The large peak at 3.2p.p.m. varied in amplitude with salinity and has been tentatively assigned to betaine. Note the small peak at 3.85p.p.m., which is also indicative of betaine.





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