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Fig. 7. Example mass spectra (one of five) of the 3.5x106 Da hemoglobin under (A) non-reducing, (B) reducing and (C) reduced and carboxyamidomethylated (Cam) conditions. (D) Model of structure of the globin monomers and dimers. The inset in A shows the linker dimers on the same intensity scale as in A but on an expanded mass scale. a1-a4, b and c, globin chain subunits; LD1-LD4, linker dimers; Dm, globin dimer; x2, portion of the spectrum where intensity has been multiplied by 2.





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