
Fig. 5. Effects of 10-5moll-1 bumetanide on (A) fluid
secretion rate, (B) K+ flux and (C) Na+ flux in
serotonin-stimulated tubules. At t=10 min, bumetanide was added to
the Malpighian tubules in the experimental group (open circles) and vehicle
(0.1% ethanol) was added to the control group (filled circles). Data are
expressed as means ± S.E.M. Asterisks indicate significant differences
(P<0.05) between the control and experimental groups; N=7
for the experimental group and N=10-12 for the controls.