Fig. 2. Northern blots (A) and analysis (B) of three mitochondrial encoded and two
nuclear encoded genes whose protein products include subunits of complex IV
(cytrochrome c oxidase) and V (ATP synthase) of the mitochondrial
electron transport chain, in four tissues from euthermic and hibernating
ground squirrels. Northern blots of total RNA (16 µg lane-1)
were scanned, then band intensities quantified and normalized to their
corresponding 28S ribosomal bands. The ratios of normalized band intensities
were then calculated. Values are mean ± S.E.M., N=3 separately
run northern blots; each blot contained RNA prepared from separate
animals.
