Fig. 3. Recordings of circadian wheel-running activity and plots of activity onsets
from cockroaches kept in constant darkness. (A,B) After injection of 200 fmol
of Mas-allatotropin in 2 nl of saline at CT15:30 h of day 16 (arrowhead),
regression analysis through consecutive activity onsets (B) revealed a phase
delay 
of 3.05 circadian hours (hCT) after the
injection. (C,D) Injection of 50 pmol of GABA in 0.5 nl of saline with blue
food dye at CT 17:30 h of day 9 (arrowhead) induced a phase advance of 3.15
circadian hours. Neither experiment had any effect on the period 
of the
activity rhythm, after a new stable state, through several transient states
(days 17-28 in A; days 10-18 in C), had been established. CT, circadian
time.
