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Fig. 2. Accumulation of the sperm attractant in sea water around live eggs. Freshly spawned eggs were suspended in filtered sea water (FSW) and incubated for 2 min or 5 min. The resulting egg-conditioned sea water (ESW) solutions were filtered to 0.22 µm and immediately bioassayed using fresh sperm. Swimming speed was quantified using computer-assisted video motion analysis. Values are means + S.E.M.; means labeled with different letters differed significantly (one-way ANOVA with post-hoc Scheffé test, P<0.005).





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