Fig. 7. Repeated intermediate-term memory (ITM) training by itself does not result in long-term memory (LTM). (A) A cohort of naïve snails (N=15) received six ITM training session (i.e. 15 min sessions separated by a 30 min rest interval). Learning was evident (ANOVA, F_14,5=14.2849, P<0.0001): session 6 was significantly different from session 1 (P<0.01); but memory was not present when tested 72 h later. That is, there was a significant difference between session 6 and the memory test (MT) (P<0.01) but no significant difference between MT and session 1 (NSD, P>0.05). (B) A naïve cohort of snails (N=20) first received three ITM training sessions and then, following a 4 h interval, received a further three ITM training sessions. In each sequence of ITM training, learning was observed (ANOVA, F_19,2=12.171, P<0.0001, in the first sequence; ANOVA, F_19,2=10.09, P<0.0003, in the second sequence). However, when LTM was tested 72 h after the last ITM training session (session 6), no memory was observed; i.e. MT was significantly different from session 6 (P<0.01) but was not significantly different from session 4 or session 1 (NSD, P>0.05 in both cases).

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