Fig. 2. The long-term memory (LTM) training procedure results in learning and memory that persists for 48 h but not for 72 h. (A) A cohort of 20 naïve snails received three 15 min operant conditioning training sessions, with each training session separated by a 1 h rest interval. Learning occurred (ANOVA, F19,2=9.7738, P<0.001); session 3 was significantly different from session 1 (P<0.01). Memory was tested 48 h later (memory test, MT; cross-hatched column). There was no significant difference between the response in the MT and that in session 3 (NSD, P>0.05), but there was a significant difference between the response in session 1 and that in the MT (P<0.01). (B) As in A, except that the memory test (MT) was presented 72 h after session 3 (N=20). Learning occurred (ANOVA, F19,2=11.4214, P<0.001); session 3 was significantly different from session 1 (P<0.01). There was no memory 72 h after the last training session. There was a significant difference between the response in session 3 and that in the MT (P<0.01), but there was no significant difference between the response in session 1 and that in the MT (NSD, P>0.05).
