Fig. 1. The intermediate-term memory (ITM) training procedure results in learning and a memory that persists for 3 h but not for 4 h. (A) A cohort of 20 naïve snails received three 15 min operant conditioning training sessions, with each training session separated by a 30 min rest interval. Learning occurred (ANOVA, F19,2=9.1613, P<0.001); session 3 was significantly different from session 1 (P<0.01). Memory was tested 3 h later (memory test, MT; cross-hatched column). There was no significant difference in the response between the MT and session 3 (NSD, P>0.05), but there was a significant difference between the response in session 1 and MT (P<0.01). (B) As in A, except that the MT was presented 4 h after session 3 (N=20). Learning occurred (ANOVA, F19,2=15.7055, P<0.001); session 3 was significantly different from session 1 (P<0.01). There was no memory 4 h after the last training session. There was a significant difference between the response in session 3 and MT (P<0.01), but there was no significant difference between the response in session 1 and MT (NSD, P>0.05).
