Fig. 4. Summary of the expression pattern of MLC and troponin isoforms in fast twitch and tonic fibres. This figure combines the information from Figs 1 and 3 to indicate the positions of each of the identified myofibrillar proteins (except MLC1x) on a silver-stained gel after SDS-PAGE. Skinned (S) and non-skinned (NS) segments of the same fibres (fibre type indicated below each lane) are shown in adjacent lanes for visualization of the bands containing soluble proteins (i.e. the bands present in non-skinned segments but absent from skinned segments). For clarity, a type 1 fibre only was used to represent the fast twitch fibres, although significant differences in the quantitative and qualitative expression of MLC and troponin isoforms did exist among the twitch fibres. Note that skinning removed a prominent soluble protein band just above the MLC1 region in both fibre types. Skinning also removed a prominent soluble protein band between MLC1f and TnCf, as well as a band below TnCT in tonic fibres. Note that this frog did not contain the MLC1x isoform in any of its fibre types.
