Fig. 2. In some frogs, a novel MLC, MLC1x, is expressed in all fibre types. (Left) SDS-PAGE (silver stained) of single fibres representing most of the different fibre types. All fibres (fibre types labeled at bottom of gel lanes) were obtained from two frogs and were typed by their MHC banding pattern by SDS-PAGE, as in Fig. 1 (not shown). (Middle) Western blots of the gel on left using monoclonal MLC antibodies T14, MF5 and F310. (Right) Schematic representation based on western analysis of the MLC composition of the fibre types from this frog and six other frogs with similar MLC composition. A unique type of MLC1, designated as MLC1x, was expressed in all fibre types, producing a strikingly different expression pattern from those seen in fibres examined in Fig. 1.
