Fig. 1. Identification of MLCs in single fibres representing the full range of fibre types in R. pipiens. (A) MHC isoform composition of single fibres was determined by SDS-PAGE. Shown are MHC bands from single fibres representing each of the major and intermediate fibre types (types 1, 1-2, 2, 2-3, 3 and tonic T). All fibres were obtained from one frog. Fibres were typed by comparison with the known positions of MHC isoforms from a standard whole muscle homogenate (anterior tibialis muscle). Fibre types, based on MHC content, are indicated at the bottom of each gel lane. (B) Analysis of MLCs in each of the MHC-typed fibres shown in A (fibre types labeled at bottom). (Left) SDS-PAGE (silver stained) of each fibre. Gel markers in leftmost lane have molecular masses (from top to bottom) of 45, 31, 21.5 and 14.5 kDa. (Middle) Western blots of the gel on left using monoclonal MLC antibodies T14, MF5 and F310. (Right) Schematic representation based on western blots of the MLC composition of the fibre types from this frog and 23 other frogs with a similar MLC composition.
