Fig. 2. Midguts were pretreated with 3.2 µmol l^–1 PLA2 in M199 medium for 10 min, and the treated guts were then used in adhesion assays. Control, midguts treated with buffer only; PLA2 alone, midguts treated with active PLA2; PLA2+pBPB, midguts treated with PLA2 in the presence of 100 µmol l^–1 p-bromophenacyl bromide (pBPB), an inhibitor of the enzyme; pBPB alone, midguts pretreated with the inhibitor pBPB only; PLA2+ZnCl₂, midguts pretreated with PLA2 in the presence of 100 µmol l^–1 ZnCl₂, an inhibitor of the enzyme; ZnCl₂ alone, midguts treated with 100 µmol l^–1 ZnCl₂. Each column is the mean of 1–2 experiments, each performed in quadruplicate. The error bars represent ± S.E.M. (N=4–8). P values for each column are as follows: PLA2 alone, P=0.0002; PLA2+pBPB, P<0.0001; pBPB alone, P=0.98; PLA2+ZnCl₂, P=0.004; ZnCl₂ alone, P=0.91.

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