Fig. 1. Intracellular pH (pHi) as a function of extracellular pH (pHe) in goldfish (A) and trout (B) hepatocytes. BCPCF-loaded hepatocytes (3x106 ml–1) were incubated in standard saline, titrated to the specified pH with HCl or NaOH, until a constant fluorescent signal was obtained. Each measurement was subsequently calibrated as described in Materials and methods. Data are from 21 (goldfish) and 15 (trout) hepatocyte preparations. The linear regression analysis equation is shown for trout hepatocytes (continuous line).
