
Fig. 7. In situ hybridization of peJK2 to lobster body tissue sections. Nuclear and cytoplasmic hybridization of peJK2 is evident as silver grains accumulated over the nuclei and tissue of the epithelium (A) and heart (B). peJK2 hybridization is confined to the nuclei of the muscle fibres (C) and the nuclei and cytoplasm of the cells within the gill lamellae (D). RNase treatment of the controls reduced probe binding to background levels (EH). cy, cytoplasm; n, nucleus; m, muscle. Bars, 20 µm (A,B,E,F); 50 µm (C,D,G,H).