Erratum
for
Cousins et al., J Exp Biol 200 (9) 1351-1362.
The Journal of Experimental Biology 204, 3113 (2001)
© 2001 The Company of Biologists Limited
Release of atrial natriuretic factor prohormone peptides 1-30, 31-67 and 99-126 from freshwater- and seawater-acclimated perfused trout (Oncorhynchus mykiss) hearts
K. L. Cousins,
A. P. Farrell,
R. M. Sweeting,
D. L. Vesely and
J. E. Keen
(1997) J. Exp. Biol. 200, 1351–1362
Fig. 7 of this article was incorrect. The correct figure is shown below. The authors wish to apologise for any inconvenience this error may have caused.
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Fig. 7. (A) Evidence that proANF 1-30 exists as a separate entity in trout plasma when plasma is subjected to high-performance gel-permeation chromatography followed by proANF 1-30 radioimmunoassay. In the evaluation of trout plasma, two peaks were seen. One of these, at approximately 9800Da, was consistent with proANF 1-98, while a second peak at fractions 68–71 was seen in the region where the pure synthetic form of proANF 1-30 (arrow) elutes. (B) ProANF 31-67 exists in trout plasma as a distinct peptide. This elution of proANF 31-67 from plasma was identical with the elution of the pure human synthetic form of proANF 31-67. (C) Atrial natriuretic factor exists as a separate entity in trout plasma. This peak in trout plasma was identical with the elution of pure synthetic human sequence of ANF (arrow). Vo, void volume of column; Vt, total volume of column. Fraction size was 0.3ml.
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