Fig. 7. Expression of genes encoding intracellular calcium-release channels in the tubule. The gel shows the result of RT-PCR from whole-fly mRNA (lanes 2,6) and tubule-specific mRNA (lanes 3,4,7,8) using primers specific to the IP₃ receptor (IP₃R) (lanes 2–4) and the ryanodine receptor (RyR) (lanes 6–8). In lanes 4 and 8, reverse transcriptase was omitted from the cDNA synthesis reaction to control for possible genomic DNA contamination. The PCR reactions give products of the expected sizes: 547base pairs (bp) for RyR, compared to 678bp from genomic DNA, due to the inclusion of two short introns (Adams et al., 2000) and 843bp for IP₃R (Sinha and Hasan, 1999). Because the 5' IP₃R primer spans an exon–intron junction, genomic DNA should not give any PCR product. Lane 1: 1kb ladder (Stratagene, La Jolla, CA, USA); Lane 5: 100bp ladder (Life Technologies).

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