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Fig. 1. Final HPLC purification of EI-14 obtained from eel gut. (A) The active fraction obtained by chromatographic steps was subjected to reverse-phase HPLC (TSK ODS-80TM column) and eluted isocratically with 26.5% acetonitrile in 5% 2-propanol and 0.1% trifluoroacetic acid (pH 2.2). The flow rate was 0.5mlmin-1. (B) Effects of purified EI-14 on atrial beating and contractile force.





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