Fig. 6. Tissue-specific and osmotic regulation of 14-3-3.a gene transcription. 14-3-3.a mRNA abundance in various tissues of Fundulus heteroclitus was measured using northern blot analysis. (A) Northern blot of 14-3-3.a from gill epithelium RNA of F. heteroclitus acclimated to FW, SW and 2xSW for 24h. Below the 14-3-3.a mRNA blot are the corresponding bands for the two rRNAs that were used for normalization. (B) Northern blot of 14-3-3.a with samples from different tissues of F. heteroclitus acclimated to SW. Below the 14-3-3.a mRNA blot are the corresponding bands for the two rRNAs that were used for normalization. (C) Quantification of 14-3-3.a mRNA expression in gill epithelium of F. heteroclitus acclimated to various salinities for 24h. Values are normalized for 14-3-3.a mRNA abundance in SW fish (=1) that represent the pre-acclimation conditions. Values are means ± S.E.M., N=4; an asterisk indicates a significant difference from SW data. (D) Quantification of the effect of salinity acclimation on 14-3-3.a mRNA abundance in different tissues of F. heteroclitus. Data are normalized for 14-3-3.a mRNA abundance in SW fish (=1). Values are means ± S.E.M., N=4, except for testes and ovary where N=2. An asterisk indicates a significant difference from SW data.
