Fig. 5. Triton X-114 extraction solubilizes glycophosphatidylinositol
(GPI)-anchored proteins. (A) Western blot probed with anti-A and anti-B
antisera. Lane 1, supernatant from phospholipase C (PLC)-treated pellicle;
lane 2, supernatant from PLC-treated pellicle in the presence of
2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate and
phenylmethanesulfonyl fluoride; lane 3, aqueous phase of Triton X-114
extraction from phase separation of pellicle proteins; lane 4, proteins from
the detergent phase of Triton X-114 extraction and phase separation of
pellicle. (B) Anti-cross-reactive-determinant developed electroblot of the
same samples as in A. The filled arrow points to surface antigens, and the
open arrow to 40-60 kDa proteins. 8 % polyacrylamide gels were used.
