Fig. 2. Proteins removed from pellicle by phospholipase C (PLC) treatment at 37 and
0°C. (A) Western blot probed with anti-A and anti-B antisera, Lane 1,
supernatant of sham-treated pellicle at 0°C; lane 2, supernatant of
PLC-treated pellicle at 0°C; lane 3, supernatant of sham-treated pellicle
at 37°C; lane 4, supernatant of PLC-treated pellicle at 37°C. (B) Same
supernatants as in A but probed with anti-cross-reacting-determinant
(anti-CRD) antiserum. The filled arrow points to surface antigens; the open
arrow points to proteins of 40-60 kDa in gradient gels ranging from 6 to 12 %
polyacrylamide.
