Fig. 1. Phospholipase treatment removes proteins with glycophosphatidylinositol
(GPI) anchors from Paramecium pellicles. (A) Western blot probed with
anti-A and anti-B antisera. Lane 1, pellet of sham-treated pellicle; lane 2,
supernatant of sham-treated pellicle; lane 3, pellet of
inositol-phosphate-specific phospholipase C (PLC)-treated pellicle; lane 4,
supernatant of PLC-treated pellicle. The filled arrow points to surface
antigens. The open arrow points to 40-60 kDa proteins. (B) Western blot probed
with anti-cross-reacting-determinant (anti-CRD) antiserum, which binds to the
cleaved GPI anchor. Lanes as in A. Gradient gels contained 8 %
polyacrylamide.
