Fig. 2. Expression of the crustacean cardioactive peptide (CCAP) transcript in the
brain of Manduca sexta. (A) Ventral view of the brain in a day 1
first-instar larva showing a cluster of three anterior pairs of cells
(arrows), one anterio-medial pair (concave arrow) and a large ventral pair in
the mid protocerebrum (arrowheads). The sixth pair of cells was located on the
dorsal side (convex arrow; out of focus). (B) Dorsal view of a brain in a day
2 fourth-instar larva. The `sixth dorsal pair' of cells is indicated by convex
arrows, and the convex arrowheads point to the two pairs of cells that started
expressing CCAP after reaching the fourth instar. (C) Posterior view of an
adult brain showing two pairs of cells (arrows). Scale bars, 50 µm.
