Fig.1. Proctolin immunoreactivity on extensor muscles and in pereion ganglion 4 of I. emarginata. (A) Immunostained varicose axons extend along the inner layer of long fibres in pereion segment 7 and pleon segment 1. Numbers indicate identified muscle fibres. Anterior is up in all parts of the figure. Scale bar, 200µm. (B) Proctolin immunostaining reveals a symmetric pair of neurones (arrowheads) in the anterior part of the ganglion in pereion segment 4. (C) Somata of efferent neurones projecting through N3 in the same ganglion, stained by retrograde labelling with Lucifer Yellow (arrowhead indicates the anterior motoneurone). (D) Double exposure of the specimen reveals the double labelled proctolin-ir motoneurone (arrowhead). Scale bar, 100µm.
