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Fig. 1. Partial cloning of the pyruvate kinase (PK) gene in rainbow trout. (A) Nucleotide and deduced amino acid sequences of the rainbow trout PK clone. Underlined letters correspond to the primer sequences. (B) Alignments of the partial amino acid deduced PK cDNA of the rainbow trout (T_PK) (GenBank accession number: AF246146) with human PK (H_PK) (GenBank accession number: P30613). Underlined letters correspond to the primer sequences. Asterisks mark amino acid residues homologous between PKs. Residues involved in the interaction with the allosteric activator fructose-1,6-bisphosphate are marked by arrows (Jurica et al., 1998).





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