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Fig. 4. [14C]CO2 incorporation in control embryos and in embryos recovering from heat shock. Control (C) and heat-shocked (HS) (22–50°C) embryos were incubated at 25°C for 2 or 4h in [14C]NaHCO3. The protein (P), nucleic acid (NA) and precursor pool (Pre) fractions were obtained as described in Materials and methods, and 14C incorporation was determined by scintillation counting. The inset shows the results for protein and nucleic acid radioactivity on an expanded scale.





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