Fig. 4. Elution profile of fed Clarias gariepinus larval extracts subjected to gel filtration chromatography on a Sephacryl S300HR column and assayed for carbamoyl phosphate synthetase (CPSase) activity as described in the text. Note differences in scaling of the axes for the larval liver elution. Whole larvae (313h post-fertilization) elution: flow rate 0.31mlmin-1, sample volume 3.5ml, protein concentration 7.3mgml-1. Larval muscle tissue (217h post-fertilization): flow rate 0.30mlmin-1, sample volume 2.4ml, protein concentration 3.9mgml-1. Larval liver tissue (217h post-fertilization): flow rate 0.33mlmin-1, sample volume 1.9ml, protein concentration 1.6mgml-1. hPF, hours post-fertilization; CP, carbamoyl phosphate.
