Fig. 8. Adenosine deaminase (ADA) and AMP deaminase (AMP) activities in serotonin-induced saliva from adult female Culex quinquefasciatus and Aedes aegypti. Individual samples of serotonin-induced mosquito saliva collected in mineral oil were transferred to 10µl of Hepes saline (10mmoll-1Hepes, pH7.2, 100mmoll-1 NaCl) and centrifuged. Enzyme levels were determined by measuring the decrease in absorbance at 265nm in microcuvettes containing 71µl of Hepes saline, 0.1mmoll-1 substrate and 4µl of the diluted saliva sample. Each salivary sample was used for one adenosine and one AMP deaminase reaction. Values are the mean + S.E.M. of five samples from each mosquito species.
