Fig. 7. Adenosine deaminase (ADA) activity left by adult female Aedes aegypti while probing an artificial feeder containing 50µmoll-1 adenosine in HS. (A) Continuous line, spectrum of a control solution exposed in a feeder for 20min; dotted line, spectrum of a solution after exposure to approximately 100 female mosquitoes for 20min. (B) Mosquito-exposed feeder solution (5µl) was diluted with 100µmoll-1 adenosine in 95µl of HS, and the resulting solution was scanned at 12min intervals. The arrows indicate the change in the spectrum over time. (C) Differential spectra of the data in B, obtained by subtracting each scan at 12min intervals from the scan at time zero. (D) Rate of change of absorbance at 265nm, used to calculate the specific activity of ADA.
