Fig. 8. Polymorphism at the 87A7 and 87C1 hsp70 gene clusters. (A) PCR products from individual flies differing at the 87A7 and 87C1 hsp70 gene clusters. Left-hand lane, DNA size markers; other lanes, PCR products from single flies of each genotype as indicated. (B) A 265bp H.M.S. Beagle long-terminal repeat (LTR) is present in the 87A7 intergenic region of the T strains. The insertion lies 391bp upstream of the 56H8/122 insertion/deletion polymorphism (triangle, 142bp; see text). The nucleotide sequence begins 161bp upstream of the H.M.S. Beagle insertion. The remainder of the 87A7 intergenic region was not sequenced: its size ranges from 1.6kb in 122-type clusters to 3.6kb in 56H8-type clusters (Bettencourt, 2001; Ish-Horowicz et al., 1979; Mason et al., 1982). (C) Site of insertion of H.M.S. Beagle element showing duplicated host DNA (bold). (D) A 1.4kb fragment corresponding to the 3' end of the Jockey element is inserted 107bp upstream of the hsp70Ba transcription start site in the T strains. The orientation of Jockey is inverted with respect to the hsp70Ba gene, with the oligo(dT) at the distal end of the fragment. The insertion intervenes between HSE 2 and HSE 3, displacing HSE 3 and HSE 4 as well as three GAGA elements (hatched boxes; defined as the pentamer consensus sequence GAGAG; Omichinski et al., 1997; Wilkins and Lis, 1997). (E) Site of insertion of Jockey. Duplicated host DNA (bold) contains one of the putative GAGA elements. (t)n, reverse complement of poly(A) tail.
