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First published online April 17, 2009
Journal of Experimental Biology 212, 1270-1276 (2009)
Published by The Company of Biologists 2009
doi: 10.1242/jeb.022764
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Intrinsic mechanical properties of the perfused armoured catfish heart with special reference to the effects of hypercapnic acidosis on maximum cardiac performance

Linda M. Hanson1,*, Daniel W. Baker1, Louise J. Kuchel1,{dagger}, Anthony P. Farrell2, Adalberto L. Val3 and Colin J. Brauner1

1 Department of Zoology, University of British Columbia, 6270 University Boulevard, Vancouver, BC, V6T 1Z4 Canada
2 Faculty of Land and Food Systems and Department of Zoology, University of British Columbia, Vancouver, V6T 1Z4 Canada
3 Laboratory of Ecophysiology and Molecular Evolution, Instituto Nacional de Pesquisas da Amazônia, Manaus, Brazil


Figure 1
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Fig. 1. The effect of changes in input pressure on cardiac output (A) and cardiac stroke volume (B) in in situ perfused Pterygoplichthys pardalis hearts under control, normocapnic conditions at 27.0±0.2°C (N=6). Results are presented as change from routine preload pressure and were normalized to a first order sigmoidal equation (r2>0.98) as discussed in the Results. Data are presented as the means for each preload value ± s.e.m.

 

Figure 2
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Fig. 2. The effect of changes in after-load on cardiac power output in in situ perfused Pterygoplichthys pardalis hearts under control, normocapnic conditions at 27.0±0.2°C (N=6). Data are presented as means ± s.e.m.

 

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Fig. 3. The effect of hypercapnia on (A) maximum cardiac power generation, POmax, (B) maximum cardiac output, Qmax, (C) cardiac stroke volume and (D) heart rate of in situ perfused Pterygoplichthys pardalis hearts at 27.0±0.2°C (N=5). Hearts were sequentially exposed to CO2 tensions of 1.0% (control), 2.5%, 5.0% and 7.5% before being returned to 1.0% CO2 to assess recovery (open circles). Hearts were then re-exposed to 7.5% CO2 to permit sampling for intracellular pH determinations (marked circles). One heart failed to recover from exposure to extreme hypercapnia and thus has been presented separately (squares).

 

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© The Company of Biologists Ltd 2009