QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

First published online February 13, 2009
Journal of Experimental Biology 212, 684-692 (2009)
Published by The Company of Biologists 2009
doi: 10.1242/jeb.026450

This Article Summary Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Wood, C. M. Articles by Walsh, P. J. Search for Related Content PubMed Articles by Wood, C. M. Articles by Walsh, P. J. Social Bookmarking                         What's this?

Using omeprazole to link the components of the post-prandial alkaline tide in the spiny dogfish, Squalus acanthias

Chris M. Wood^1,4,5,*, Aaron G. Schultz^2,5, R. Stephen Munger^1,5 and Patrick J. Walsh^3,4,5

¹ Department of Biology, McMaster University, 1280 Main St. West, Hamilton, Ontario, Canada L8S 4K1
² School of Life and Environmental Sciences, Deakin University, Pigdons Road, Geelong, 3217, Australia
³ Department of Biology, University of Ottawa, 30 Marie Curie, Ottawa, Ontario, Canada K1N 6N5
⁴ Rosenstiel School of Marine and Atmospheric Sciences, University of Miami, Miami, FL 33149, USA
⁵ Bamfield Marine Sciences Centre, 100 Pachena Drive, Bamfield, British Columbia, Canada V0R 1B0

View larger version (7K): [in this window] [in a new window]   Fig. 1. Changes in the pH of gastric chyme in series 1 following an involuntary meal administered by stomach tube at 0 h in dogfish pre-treated with omeprazole (open circles) or vehicle (filled circles), relative to the pH values of the external seawater and the meal. *Significant difference between the two treatment groups at the same sample time; significant difference from the external seawater (SW); significant difference from the original meal; all at P<0.05. Within a treatment group, means sharing letters of the same case are not significantly different from one another. Means ±1 s.e.m. (omeprazole: N=11, 7, 7 and 21 at 12 h, 24 h, 36 h and 48 h, respectively; vehicle: N=9, 7, 5 and 15 at 12 h, 24 h, 36 h and 48 h, respectively).

View larger version (13K): [in this window] [in a new window]   Fig. 2. Changes in (A) sodium concentration, (B) chloride concentration and (C) potassium concentration of gastric chyme in series 1 following an involuntary meal administered by stomach tube at 0 h in dogfish pre-treated with omeprazole (open circles) or vehicle (filled circles), relative to the concentrations in the external seawater (SW) and the meal. *Significant difference between the two treatments groups at the same sample time; significant difference from the SW; significant difference from the original meal; all at P<0.05. Within a treatment group, means sharing letters of the same case are not significantly different from one another. Means ±1 s.e.m. (N numbers as in legend of Fig. 1).

View larger version (13K): [in this window] [in a new window]   Fig. 3. Changes in (A) magnesium concentration, (B) calcium concentration and (C) total ammonia concentration of gastric chyme in series 1 following an involuntary meal administered by stomach tube at 0 h in dogfish pre-treated with omeprazole (open circles) or vehicle (filled circles), relative to the concentrations in the external seawater (SW) and the meal. *Significant difference between the two treatments groups at the same sample time; significant difference from the SW; significant difference from the original meal; all at P<0.05. Within a treatment group, means sharing letters of the same case are not significantly different from one another. Means ±1 s.e.m. (N numbers as in legend of Fig. 1).

View larger version (15K): [in this window] [in a new window]   Fig. 4. Changes in (A) arterial pH (pHa), (B) arterial plasma bicarbonate concentration [HCO3–] and (C) arterial partial pressure of carbon dioxide (PaCO2) following an involuntary meal administered by stomach tube at 0 h in dogfish pre-treated with omeprazole (open circles) or vehicle (filled circles) in series 2. *Significant difference from the pre-feeding control value in the respective treatment group; significant difference between the two treatment groups at the same sample time; both at P<0.05. Means ±1 s.e.m. (omeprazole: N=8; vehicle N=9 at each time).

View larger version (9K): [in this window] [in a new window]   Fig. 5. Changes in the net excretion rates of basic equivalents to the external seawater following an involuntary meal administered by stomach tube at 0 h in dogfish pre-treated with omeprazole (open circles) or vehicle (filled circles) in series 3. Positive values indicate net base excretion, negative values indicate net acid excretion. *Significant difference from the pre-feeding control value in the respective treatment group; significant difference between the two treatment groups at the same sample time; both at P<0.05. Means ±1 s.e.m. (omeprazole: N=9; vehicle N=9 at each time).

CiteULike

Complore

Connotea

Del.icio.us

Digg

Reddit

Technorati

Twitter