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First published online January 30, 2009
Journal of Experimental Biology 212, 542-549 (2009)
Published by The Company of Biologists 2009
doi: 10.1242/jeb.022889
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The specific binding sites of eyestalk- and pericardial organ-crustacean hyperglycaemic hormones (CHHs) in multiple tissues of the blue crab, Callinectes sapidus

Hidekazu Katayama* and J. Sook Chung{dagger}

University of Maryland Biotechnology Institute, 701 E. Pratt Street, Columbus Center, Suite 236, Baltimore, MD 21202, USA


Figure 1
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  		Fig. 1. Spatial distribution of specific binding sites of 125I-ES-CHH (A) and 125I-PO-CHH (B). (A) The specific binding sites of 125I-ES-CHH were challenged with 10 pmol of cold ES-CHH (filled bars) or cold rPO-CHH (open bars). (B) The specific binding sites of 125I-PO-CHH were competed for with 10 pmol of cold rPO-CHH (open bars) or cold ES-CHH (filled bars). Data are presented as means ±1 s.e.m. (N=6).

 

Figure 2
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  		Fig. 2. Saturation curves and Scatchard plots (insets) of the specific binding of 125I-ES-CHH in the membranes of hepatopancreas (A) and gills (B). Displacement curves (C) of specific binding in gills by ES-CHH (1) and in hepatopancreas by ES-CHH (2), oxidized ES-CHH (4), C. maenas ES-CHH (3) and C-terminal synthetic peptide of ES-CHH (5).

 

Figure 3
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  		Fig. 3. Saturation curves and Scatchard plots (insets) of the specific binding of 125I-rPO-CHH (A) or 125I-ES-CHH (B) in the abdominal muscle membranes. Displacement curves (C) of specific binding of 125I-rPO-CHH by rPO-CHH (open circles), and by ES-CHH (filled circles); displacement curves of specific binding of 125I-ES-CHH by ES-CHH (filled triangles) and by rPO-CHH (open triangles).

 

Figure 4
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  		Fig. 4. The effects of ES-CHH (50 nmol l–1) and rPO-CHH (100 nmol l–1) incubation on cGMP production in various tissues: open bars, ES-CHH; filled bars, rPO-CHH. The data were converted into fold increase over control that was only treated with saline, and are presented as means ±1 s.e.m. (N=6). Solid line represents the level of control for ES-CHH and dotted line that for rPO-CHH.

 

Figure 5
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  		Fig. 5. Time course effects of CHH neuropeptides on the level of glucose in haemolymph in vivo: (open circles) rPO-CHH at 150–200 pmol (filled squares) native ES-CHH at 10 pmol, and (filled circles) saline control. The data are presented as means ±1 s.e.m. (N=18) of the percentage increase over control at t=0 min. Student's t-test was used for statistical analysis of each experiment. *P<0.05, **P<0.01, ***P<0.001. The data obtained from each time point of three experimental groups were tested using one-way ANOVA at P<0.05. Statistical significance was shown in lowercase letters.

 

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