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First published online January 30, 2009
Journal of Experimental Biology 212, 494-498 (2009)
Published by The Company of Biologists 2009
doi: 10.1242/jeb.022780
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Increase in Presenilin 1 (PS1) levels in senescence-accelerated mice (SAMP8) may indirectly impair memory by affecting amyloid precursor protein (APP) processing

Vijaya B. Kumar*, Mark Franko, William A. Banks, Pranav Kasinadhuni, Susan A. Farr, Kamlesh Vyas{dagger}, Veena Choudhuri{ddagger} and John E. Morley

Division of Geriatric Research, Education and Clinical Center, VA Medical Center, St Louis, MO 63125, USA and St Louis University Health Sciences Center, St Louis, MO 63104, USA


Figure 1
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  		Fig. 1. Comparison of the amino acid sequence of PS1 of SAMP8 with that of house mouse, human and rat. Shaded regions represent the mutations linked to familial Alzheimer's disease (FAD) in human sequences reported thus far. Common sequences are represented by hyphens. Glycosylation sites are represented by bold letters, and transmembrane regions are underlined. Arrows show the substitution of specified amino acids.

 

Figure 2
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  		Fig. 2. Western blots of hippocampal PS1 from various age groups of SAMP8 mice. The hippocampal tissue was homogenized and subjected to western blotting as described in the text. PS1 was detected using anti-PS1 polyclonal antibody (A). The same blot was stripped for 10 min and re-probed with GAPDH monoclonal antibody (B).

 

Figure 3
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  		Fig. 3. Quantification of hippocampal PS1 of different age groups of SAMP8 mice: (A) holoprotein; (B) heterodimer. The bands obtained in Fig. 2 were scanned and quantified using UnScan it program (Silk Scientific, Orem, UT, USA). The total density obtained in each band is expressed as the number of pixels. The ratio of densities of PS1 and GAPDH bands is plotted against the age of the mouse. P-value by one-way ANOVA between age groups is <0.02 (*) and <0.031 (**). The change in holoprotein level normalized to GAPDH is 0.17±0.02 to 0.28±006.

 

Figure 4
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  		Fig. 4. Western blotting of hippocampal PS1 from various age groups of CD-1 mice. The hippocampal tissue was homogenized and subjected to western blotting as described in the text. PS1 was detected using anti-PS1 polyclonal antibody (A). The same blot was stripped for 10 min and re-probed with GAPDH monoclonal antibody (B).

 

Figure 5
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  		Fig. 5. Quantification of hippocampal PS1 from various age groups of CD-1 mice: (A) holoprotein; (B) heterodimer. The bands obtained in Fig. 4 were scanned and quantified as in Fig. 3. The total density obtained in each band is expressed as the number of pixels. The ratio of densities of PS1 and GAPDH bands is plotted against the age of the mouse. P, determined by one-way ANOVA, is <0.17 (*) and <0.013 (**). The change in the holoprotein levels normalized to GAPDH is 1.14±0.12 to 0.71±0.21.

 

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