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First published online May 1, 2009
Journal of Experimental Biology 212, 1553-1558 (2009)
Published by The Company of Biologists 2009
doi: 10.1242/jeb.022210
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The role of insulin and glucose in goose primary hepatocyte triglyceride accumulation

Chunchun Han, Jiwen Wang*, Liang Li, Zhongxian Zhang, Li Wang and Zhixiong Pan

Key Lab of Animal Genetic Resources, College of Animal Science and Technology, Sichuan Agricultural University, Ya'an, Sichuan 625014, P.R.C.


Figure 1
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  		Fig. 1. Triglyceride (TG) accumulation in geese hepatocytes after 0–96 h in the presence of 30 mmol l–1 glucose or no glucose as control. Different lowercase letters indicate a significant difference between treatments (P<0.05). After 24 h in serum-free medium, hepatocytes were incubated in the presence of 30 mmol l–1 glucose or no glucose (control) for 24, 48, 72 and 96 h.

 

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  		Fig. 2. Relative mRNA levels of SREBP-1, ACC{alpha} and FAS in goose primary hepatocytes treated with different concentrations of insulin. Different lowercase letters indicate a significant difference between treatments (P<0.05). After 24 h in serum-free medium, hepatocytes were incubated for 72 h either with no addition as a control or in the presence of 50, 100, 150 or 200 nmol l–1 insulin.

 

Figure 3
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  		Fig. 3. Immunoblot analysis of nuclear SREBP-1 in goose hepatocyte nuclear extracts treated with insulin and glucose. (A) control, (B) 50 nmol l–1 insulin, (C) 5 mmol l–1 glucose, (D) 50 nmol l–1 insulin + 5 mmol l–1 glucose. After 24 h in serum-free medium, hepatocytes were incubated for 48 h with insulin and glucose. The standard culture medium contained 25 mmol l–1 glucose before additions. Each blot is representative of two independent experiments. Different lowercase letters indicate differences between treatments (P<0.05).

 

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  		Fig. 4. EMSA performed with goose hepatocyte nuclear extracts treated with insulin and glucose. (A) control, (B) 50 nmol l–1 insulin, (C) 5 mmol l–1 glucose, (D) 50 nmol l–1 insulin + 5 mmol l–1 glucose. After 24 h in serum-free medium, hepatocytes were incubated for 48 h with insulin and glucose. The standard culture medium contained 25 mmol l–1 glucose before additions. Each blot is representative of two independent experiments.

 

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© The Company of Biologists Ltd 2009