First published online May 1, 2009
Journal of Experimental Biology 212, 1436-1441 (2009)
Published by The Company of Biologists 2009
doi: 10.1242/jeb.028951
Increased locomotor activity and metabolism of Aedes aegypti infected with a life-shortening strain of Wolbachia pipientis
Oliver Evans1,
Eric P. Caragata1,
Conor J. McMeniman1,
Megan Woolfit1,
David C. Green2,
Craig R. Williams3,
Craig E. Franklin1,
Scott L. O'Neill1 and
Elizabeth A. McGraw1,*
1 School of Biological Sciences, The University of Queensland, St Lucia,
Queensland, 4072, Australia
2 Information Technology Services, The University of Queensland, St Lucia,
Queensland, 4072, Australia
3 Sansom Institute, University of South Australia, GPO Box 2471, Adelaide, South
Australia, 5001, Australia

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Fig. 1. Mosquito observation chamber with 10 individual cells constructed with
white opaque plastic. Cells are covered with a sliding door of transparent
plastic for videography.
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Fig. 2. Mean total time active (±s.e.m.) per 1 h window for infected and
uninfected males and females at three adult ages. Times on x-axis
denote the beginning of the hour-long session. Lights were turned on daily at
07:00 h and off at 19:00 h. Each point represents 10 mosquitoesx3
replicate recording days.
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Fig. 3. Mean metabolic rate (±s.e.m.) based on two 4 h windows
(07:30–11:30 h and 11:30–15:30 h) for infected (black bars) and
uninfected (white bars) males and females at three adult ages. Each bar
represents data from 15 mosquitoesx3 replicatesx2 windows.
*P<0.05, **P<0.01.
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© The Company of Biologists Ltd 2009