spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

First published online April 18, 2008
Journal of Experimental Biology 211, 1456-1462 (2008)
Published by The Company of Biologists 2008
doi: 10.1242/jeb.012328
This Article
Right arrow Summary Freely available
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rey, B.
Right arrow Articles by Voituron, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rey, B.
Right arrow Articles by Voituron, Y.
Social Bookmarking
Add to CiteULike   Add to Complore   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati   Add to Twitter  
What's this?

Reptilian uncoupling protein: functionality and expression in sub-zero temperatures

Benjamin Rey1, Brigitte Sibille1, Caroline Romestaing1, Maud Belouze1, Dominique Letexier1, Stéphane Servais1, Hervé Barré1, Claude Duchamp1 and Yann Voituron2,*

1 Laboratoire de Physiologie Intégrative, cellulaire et moléculaire, Centre National de la Recherche Scientifique (CNRS) – Université Claude Bernard Lyon 1, 43 Bvd 11 Novembre 1918, F-69622 Villeurbanne Cedex, France
2 Station d'Ecologie Expérimentale du CNRS à Moulis, Laboratoire Evolution et Diversité du Vivant, Moulis, F-09200 Saint-Girons, France


Figure 1
View larger version (39K):
[in this window]
[in a new window]

  		Fig. 1. Partial predicted amino acid sequence and alignment of repUCP with other homologues of mammalian UCPs. The asterisks indicate amino acid preserved in all presented UCPs. A black background indicates the nucleotides that are essential for GDP binding (Arg83, Arg182 and Hist214) and that they are present in all the mammalian UCP homologues.

 

Figure 2
View larger version (8K):
[in this window]
[in a new window]

  		Fig. 2. Tissue distribution of repUCP mRNA (390 bp) in Lacerta vivipara after 32 cycles of PCR. Lane 1 is a 100 pb ladder, lanes 2–7 show expression of reptilian UCP in liver, skeletal muscle, lung, heart, brain and adipose tissue, respectively, from one representative individual.

 

Figure 3
View larger version (18K):
[in this window]
[in a new window]

  		Fig. 3. RT-PCR analysis of (A) PGC-1{alpha}, (B) PPAR{gamma} and (C) reptilian UCP mRNA levels in muscle from controls, supercooled, frozen and thawed lizards (Lacerta vivipara). For details, see Materials and methods. β-actin was used as an invariant gene. Values are means ± s.e.m. for eight lizards in each group. Different letters indicate a significant difference (P<0.05).

 

Figure 4
View larger version (9K):
[in this window]
[in a new window]

  		Fig. 4. Representative experimental traces of oxygen consumption by muscle mitochondria from control lizard energised with succinate (for details, see Materials and methods). (A) Mitochondrial state 4, state 3 (addition of 1 mmol l–1 ADP) and state 4+ (addition of 1 µg ml–1 oligomycin). (B) Effect of two sequential additions of 1 mmol l–1 GDP on state 4 mitochondrial oxygen consumption.

 

Add to CiteULike CiteULike   Add to Complore Complore   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati   Add to Twitter Twitter    What's this?



© The Company of Biologists Ltd 2008